Journal: Frontiers in Pharmacology
Article Title: Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promoted Wound Healing by Regulating PI3K/AKT/mTOR and SIRT1/NF-κB Pathways
doi: 10.3389/fphar.2022.913408
Figure Lengend Snippet: Immunohistochemistry staining of wound tissues on days 7 and 14. (A) Representative images for CD31, PCNA, α-SMA, and CD68 staining (scale bar = 100 μm). (B–E) Quantification of CD31, PCNA, α-SMA, and CD68 protein expressions, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001 vs . control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs . CAH group as statistically significant.
Article Snippet: The primary antibody against CD31 (ab182981) came from Abcam (Cambridge, United Kingdom); PCNA (BM0104), α-SMA (BM0002), and CD68 (BA3638) were from BOSTER (Wuhan, China); p-PI3K (#4228) was from Cell Signaling Technology (Beverly, MA, United States); p-AKT (ARG51559) and p-mTOR (ARG40666) were acquired from Arigo (Hsinchu, Taiwan, China); and PI3K (67071-1-lg), AKT (60203-2-lg), mTOR (10745-1-AP), SIRT1 (13161-1-AP), NF-κB (p65, 10745-1-AP), IL-1β (26048-1-AP), TNF-α (17590-1-AP), β-actin (66009-1-lg), and horseradish peroxidase (HRP)–conjugated second antibodies (SA00001-1/SA00001-2) were purchased from Proteintech Group (Chicago, IL, United States).
Techniques: Immunohistochemistry, Staining, Control